Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Krishanender, Dinesh
- Nucleotide Sequence Variation in Exons 9 and 10 of Lactoferrin Gene in Murrah Buffalo
Abstract Views :212 |
PDF Views:1
Authors
Affiliations
1 Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal - 132 001, IN
1 Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal - 132 001, IN
Source
Himachal Journal of Agricultural Research, Vol 44, No 1&2 (2018), Pagination: 80-84Abstract
The study was undertaken with the objectives to characterize and identify single nucleotide polymorphisms in exons 9 and 10 of lactoferrin gene in Murrah buffalo. Genomic DNA was isolated from 200 lactating Murrah buffaloes and exons 9 and 10 of lactoferrin gene were amplified using specific sets of primers, which resulted in amplicons of 332 and 209 bp respectively. Comparison of nucleotide sequence of exonic region of lactoferrin gene in Murrah buffalo with that of Bos taurus cattle revealed a total of 16 mutations comprising of 11 transition and 5 transversion type. Conceptualized translation of nucleotide sequence of exonic region revealed six amino acid changes (five in exon 9 and one in exon 10). The BLAST analysis revealed that exons 9 and 10 of lactoferrin gene in Murrah buffalo were 94 to 99 % identical with other species. The identified SNPs may serve as marker for selection of Murrah buffaloes for improved resistance to mastitis.Keywords
Lactoferrin, Murrah Buffalo, SNP.Full Text
References
- Joshi S and Gokhale S 2006. Status of mastitis as an emerging disease in improved and peri urban dairy farms in India.Annals of the New York Academy of Sciences 74-83.
- Kathiravan Periasamy, Kataria RS, Mishra Bishnu P, Dubey Praveen K, Selvakumar M and Tyagi Neetu. 2009. Seven novel single nucleotide polymorphisms identified within river buffalo (Bubalus bubalis) lactoferrin gene. Tropical Animal Health Production 42:1021–1026.
- Kimber I, Cumberbatch M, Dearman DR, Headon DR, Bhushan M and Griffiths CEM. 2002. Lactoferrin: influences on Langerhans cells, epidermal cytokines, and coetaneous inflammation. Biochemistry and Cell Biology 80: 103-107.
- Li G-H, Zhang Y, Sun D-X and Li N. 2004. Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis. Animal Biotechnology 15: 67-76.
- O'Halloran FB, Bahar B, Buckley F, Sullivan O O, Sweeney T and Giblin L. 2009. Characterization of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds. Biochimie 91: 68-75.
- Raja KN. 2007. Characterization of bovine lactoferrin gene and its polymorphism in Sahiwal cattle. Ph. D. thesis submitted to N.D.R.I., Karnal. Sambrook J and Russell DW. 2001. 3rd edition. Cold Spring Harbor Laboratory Press, New York, 6.16.62.
- Sanchez L, Calvo M and Brock JH. 1992. Biological role of lactoferrin. Archives of D Reiter, B 1985. Protective proteins in milk–biological significance and exploitation. Bulletin of International Dairy Federation 191: 1-35.
- Seyfert HM and Kuhn C. 1994. Characterization of a first bovine lactoferrin gene variant based on an EcoRI polymorphism. Animal Genetics 25: 54.
- Teng CT. 2002. Lactoferrin gene expression and regulation: an overview. Biochemistry and Cell Biology 80: 7-16.